bibo:abstract
  • The assessment of the degree of restriction fragment length polymorphism (RFLP) may facilitate construction of RFLP linkage maps. For the four parents derived from two soybean populations, 'Suwon 157'x'Danbaekkong' and 'Pureunkong'x'Jinpumkong 2', the degree of RFLP was assessed by screening parental genomic DNA digested with each one of five restriction enzymes (DraⅠ, EcoRⅠ, EcoRⅤ, HindⅢ, or TaqⅠ) using cDNA and/or genomic clones from five legume species (Glycine max, Vigna radiata, Phaseolus vulgaris, Arachis hypogaea, and Medicago sativa). The restriction enzymes, EcoRⅠ and HindⅢ, detected higher polymorphisms between parents in both populations. Comparatively higher polymorphisms were found using DNA probes from G. max, A. Hypogaea, and P. vulgaris. Of 246 DNA probes, 58% detected polymorphisms between 'Suwon 157' and 'Danbaekkong', and 51% between 'Pureunkong' and 'Jinpumkong 2', indicating the higher level of polymorphisms. This higher polymorphism in both populations is probably due to the larger amount of genetic diversity between soybean genotypes evaluated in this study. (rdf:langString) (en)
  • 수원 157호와 단백콩, 푸른콩과 진품콩 2호간의 두 F₂ 분리집단으로 유전자 지도를 작성하기 위한 기초단계로, 모부본 4품종의 게놈 DNA를 5종의 DNA 제한효소 (DraⅠ, EcoRⅠ, EcoRⅤ, HindⅢ, TaqⅠ)로 절단하여, 콩, 녹두, 강낭콩, 땅콩, 알팔파의 cDNA 혹은 genomic clone으로 제조된 246종의 DNA 인자를 사용하여 Southern blot 분석으로 RFLP 다양성을 조사하였다. 1. 다양한 두과작물로 부터 유래한 DNA인자를 이용함으로써, 제한효소로 절단된 콩 DNA의 RFLP 인식에 유용하였다. 2. 수원 157호와 단백콩 간 RFLP를 인식한 DNA 인자의 86.4%, 푸른콩과 진품콩 2호간 80.0%가 한 개의 유전자좌를 감지하였다. 3. DNA 제한효소 가운데 EcoRI과 HindⅢ가 다른 제한효소에 비하여 RFLP 출현율이 비교적 높았다. 4. 수원 157호 및 단백콩간에 RFLP 출현율은 58%, 푸른콩 및 진품콩 2호간에는 51%를 나타내어 지금까지 보고된 미국콩보다도 그 출현율이 높았다. (rdf:langString) (ko)
nlon:biographicalNote
  • Seok Dong Kim, National Crop Experiment Station (xsd:string)
  • Suk Ha Lee, National Crop Experiment Station (xsd:string)
  • Yong Hwan Ryu, National Crop Experiment Station (xsd:string)
nlon:classificationNumberOfNLK
  • 523.205 (xsd:string)
nlon:containedIn
dc:creator
  • Kim, Seok Dong (xsd:string)
  • Ryu, Yong Hwan (xsd:string)
  • 이석하 (xsd:string)
dcterms:creator
nlon:datePublished
  • 2021-01-30T23:37:12 (xsd:dateTime)
dcterms:description
  • 콩의 RFLP 다양성 (xsd:string)
bibframe:extent
  • 28 cm (xsd:string)
  • p. 210-215 (xsd:string)
dcterms:isPartOf
nlon:issuedYear
  • 1997 (xsd:string)
nlon:itemNumberOfNLK
  • 한575 (xsd:string)
rdfs:label
  • Diversity of restriction fragment length polymorphism in soybean / Suk Ha Lee, Yong Hwan Ryu,Seok Dong Kim (xsd:string)
dcterms:language
dc:subject
  • DNA probes (xsd:string)
  • Genetic diversity (xsd:string)
  • RELP (xsd:string)
  • Soybean (xsd:string)
  • 다양성 (xsd:string)
  • (xsd:string)
dcterms:title
  • Diversity of restriction fragment length polymorphism in soybean (xsd:string)
rdf:type
nlon:volumeOfNLK
  • 29(1) (xsd:string)