bibo:abstract
  • The protein from soybean seeds were examined by polyacrylamide gel electrophoresis and the trypsin inhibitor(T.I) bands were detected. The water-extractable protein was fractionated into three fractions by Sephadex G-75 gel filtration. The T.I. activity was found only in the second fraction. Kunitz and Bowman-Birk inhibitors were fractionated by DEAE-cellulose chromatography into seven and six and six fractions, respectively. In kunitz inhibitor, 5 fractions were found to have T,I, activity and 4 fractions in Bowman-Birk inhibitor. From the band patterns of the protein extracts and those of DEAE-cellulose chromatographic fractions, it was found that band 3 and 4 were T.I. band, corresponding to T<TEX>$$i^1$$</TEX> and T<TEX>$$i^2$$</TEX> band, respectively, In addition, band 6 and 10 were presumed to be T.I. band. Of the 42 vatieties sampled, 35 revealed only T<TEX>$$i^1$$</TEX> band and 7 only T<TEX>$$i^2$$</TEX> band. The T.I. band patterns were not changed by the culture condition. The T.I. content, when expressed as the number of trypsin units inhibited(T.U.I), showed remarkable differences from 25 to 76 between varieties. The seedtime and fertilization condition had no effect on the T.I. content. Judged from the results of <TEX>$$F_1$$</TEX> seeds analysis, we assumed that T<TEX>$$i^1$$</TEX> and T<TEX>$$i^2$$</TEX> band were controlled by codominant allele at a single locus. (rdf:langString) (en)
dcterms:alternative
  • Soynean trypsin inhibitors electrophoretic differences among varieties and their fractionation on DEAE-cellulose (xsd:string)
nlon:biographicalNote
  • 김수일, 서울대학교 농과대학 농화학과 (xsd:string)
  • 나지영, 서울대학교 농과대학 농화학과 (xsd:string)
  • 문항식, 서울대학교 농과대학 농화학과 (xsd:string)
  • 이석하, 서울대학교 농과대학 농학과 (xsd:string)
  • 이홍석, 서울대학교 농과대학 농학과 (xsd:string)
nlon:classificationNumberOfNLK
  • 520.5 (xsd:string)
nlon:containedIn
dc:creator
  • 김수일 (xsd:string)
  • 나지영 (xsd:string)
  • 문항식 (xsd:string)
  • 이석하 (xsd:string)
  • 이홍석 (xsd:string)
dcterms:creator
nlon:datePublished
  • 2021-01-30T23:37:12 (xsd:dateTime)
dcterms:description
  • Search for trypsin inhibitor deficient soybean varieties and their genetic studies, Soynean trypsin inhibitors electrophoretic differences among varieties and their fractionation on DEAE-cellulose (xsd:string)
bibframe:extent
  • 26 cm (xsd:string)
  • p. 107-121 (xsd:string)
dcterms:isPartOf
nlon:issuedYear
  • 1985 (xsd:string)
nlon:itemNumberOfNLK
  • 서793ㄴ (xsd:string)
rdfs:label
  • Trypsin inhibitor 결여 大豆品種의 탐색 및 그의 遺傳育種學的 硏究 / 李弘石, 李錫河,文亢植,羅志英,金秀一 (xsd:string)
dcterms:language
nlon:remainderOfTitle
  • Trypsin inhiabitors의 전기영동 검정방법에 의한 대두 품종별 비교 및 DEAE-cellulose에 의한 분리 (xsd:string)
dc:subject
  • DEAE-cellulose (xsd:string)
  • Deficient (xsd:string)
  • Electrophoretic (xsd:string)
  • Fractionation (xsd:string)
  • Genetic (xsd:string)
  • Soybean (xsd:string)
  • Trypsin inhibitor (xsd:string)
  • Varieties (xsd:string)
  • 검정방법 (xsd:string)
  • 대두 (xsd:string)
  • 대두품종 (xsd:string)
  • 유전육종학 (xsd:string)
  • 전기영동 (xsd:string)
  • 품종 (xsd:string)
dcterms:title
  • Trypsin inhibitor 결여 大豆品種의 탐색 및 그의 遺傳育種學的 硏究 (xsd:string)
rdf:type
bibo:volume
  • 1 (xsd:string)
nlon:volumeOfNLK
  • 10(1-1) (xsd:string)