bibo:abstract
  • We analyzed the genetic variation and phylogenetic relationship among fourteen taxa of Genus Abies distributed in Korea using random amplified polymorphic DNA(RAPD) and internal transcribed spacer(ITS) 2 sequences of the nuclear ribosomal DNA(rDNA). RAPDs were used for identification of selected taxa by amplification using single 10-mer primers of arbitrary sequence. Fourteen taxa were clearly classified with 10 arbitrary primers, which generated 102 useful polymorphic bands that were evaluated according to the presence or absence of the fragments of equal size. The genetic distance between A. koreana for. chlorocarpa and A. koreana for. rubrocarpa was 0.127, showing the closest among any other pairs. By comparing the base sequences in the ITS2 region of the nuclear rDNA, genetic relationship was assesed among selected taxa. It showed twenty six sites of point mutation(base substitution) in 243 base pairs(bp) of ITS2 region. The G+C contents of ITS2 region were ranged from 58.3% to 61.7%. The value of nucleotide divergence among selected taxa was ranged from 0.4% to 9.8%. These results showed that RAPD and ITS2 sequence analysis was a useful tool for elucidating phylogenetic relationship and selection of specific marker in Abies species. (rdf:langString) (en)
nlon:biographicalNote
  • 고미희, 제주대학교 생물학과 (xsd:string)
  • 고석찬, 제주대학교 생물학과 (xsd:string)
  • 김문홍, 제주대학교 생물학과 (xsd:string)
  • 오문유, 제주대학교 생물학과, mike (rdf:langString) (cheju.cheju.ac.kr)
  • 오유성, 제주대학교 생물학과 (xsd:string)
  • 정용환, 제주대학교 생물학과 (xsd:string)
  • 한상현, 제주대학교 생물학과 (xsd:string)
nlon:classificationNumberOfNLK
  • 405 (xsd:string)
nlon:containedIn
dc:creator
  • 고미희 (xsd:string)
  • 고석찬 (xsd:string)
  • 김문홍 (xsd:string)
  • 오문유 (xsd:string)
  • 오유성 (xsd:string)
  • 정용환 (xsd:string)
  • 한상현 (xsd:string)
dcterms:creator
nlon:datePublished
  • 2021-01-30T23:37:12 (xsd:dateTime)
dcterms:description
  • Genetic variation and phylogenetic analysis of genus Abies distributed in Korea based on RAPD and nuclear rDNA ITS2 sequences (xsd:string)
bibframe:extent
  • 27 cm (xsd:string)
  • p. 193-206 (xsd:string)
dcterms:isPartOf
nlon:issuedYear
  • 2001 (xsd:string)
nlon:itemNumberOfNLK
  • 제578ㄱ (xsd:string)
rdfs:label
  • RAPD와 핵 리보솜 DNA ITS2 염기서열에 의한 한국산 전나무속 식물의 유전적 변이 및 계통유전학적 분석 / 정용환, 한상현,오유성,고미희,고석찬,김문홍,오문유 (xsd:string)
dcterms:language
dc:subject
  • Abies (xsd:string)
  • DNA (xsd:string)
  • ITS (xsd:string)
  • ITS2 (xsd:string)
  • Phylogenetic (xsd:string)
  • RAPD (xsd:string)
  • Relationship (xsd:string)
  • Variation (xsd:string)
  • rDNA (xsd:string)
  • 염기서열 (xsd:string)
  • 전나무 (xsd:string)
  • 핵 리보솜 (xsd:string)
dcterms:title
  • RAPD와 핵 리보솜 DNA ITS2 염기서열에 의한 한국산 전나무속 식물의 유전적 변이 및 계통유전학적 분석 (xsd:string)
rdf:type
nlon:volumeOfNLK
  • 14(2) (xsd:string)