bibo:abstract
  • We analyzed the mitochondrial DNA(mtDNA) cytochrome b(CytB) gene in Jeju native horses using polymerase chain reaction(PCR) mediated restriction fragment length polymorphism(RFLP). To acquire the complete sequences for the mtDNA CytB, we designed two primers based on those of prevously reported mitochondrion genome of E. caballus. The amplified PCR products were digested with 7 restriction enzymes(BamHⅠ, HaeⅢ, HindⅡ, MspⅠ, RsaⅠ, PstⅠ and TaqⅠ). We found two polymorphic patterns digested by MspⅠ and RsaⅠ, respectively, and the frequencies showed different between two populations of Jeju native horses. The morphs of the CytB in Jeju native horses divided into three types. This result showed similar to the previous molecular reports, but the frequencies of variations and the polymorphic position were different these results showed that PCR-RELP of the mtDNA CytB is useful for detecting polymorphism and verifying the lineage of Jeju native horses. (rdf:langString) (en)
nlon:biographicalNote
  • 오문유, 제주대학교 생물학과 (xsd:string)
  • 오유성, 제주대학교 생물학과 (xsd:string)
  • 정용환, 제주대학교 생물학과 (xsd:string)
  • 한상현, 제주대학교 생물학과 (xsd:string)
nlon:classificationNumberOfNLK
  • 405 (xsd:string)
nlon:containedIn
dc:creator
  • 오문유 (xsd:string)
  • 오유성 (xsd:string)
  • 정용환 (xsd:string)
  • 한상현 (xsd:string)
dcterms:creator
nlon:datePublished
  • 2021-01-30T23:37:12 (xsd:dateTime)
dcterms:description
  • Genetic polymorphism of mitochondrial cytochrome b gene in Jeju native horses inferred from PCR-RFLP (xsd:string)
bibframe:extent
  • 27 cm (xsd:string)
  • p. 39-46 (xsd:string)
dcterms:isPartOf
nlon:issuedYear
  • 2001 (xsd:string)
nlon:itemNumberOfNLK
  • 제578ㄱ (xsd:string)
rdfs:label
  • PCR-RFLP에 의한 제주 재래마 미토콘드리아 cytochrome b 유전자의 유전적 다형현상 / 한상현, 정용환,오유성,오문유 (xsd:string)
dcterms:language
dc:subject
  • Jeju native horse (xsd:string)
  • Polymorphism (xsd:string)
  • RFLP (xsd:string)
  • mtDNA CytB (xsd:string)
  • 미토콘드리아 (xsd:string)
  • 유전자 (xsd:string)
  • 재래마 (xsd:string)
dcterms:title
  • PCR-RFLP에 의한 제주 재래마 미토콘드리아 cytochrome b 유전자의 유전적 다형현상 (xsd:string)
rdf:type
nlon:volumeOfNLK
  • 14(1) (xsd:string)