bibo:abstract
  • Sessile organisms such as the oyster Crassostrea gigas have been given much attention as a potential biomonitoring indicator to assess the impact of toxicants on aquatic organism. In this study, we exposed cells isolated from gill of oyster (Crassostrea gigas) to hydrogen peroxide in vitro. In addition oysters were in vivo exposed to pyrene and benzo(a)pyrene at various concentrations for 2 weeks. Comet assay was used to detect DNA single strand breaks and to investigate the application of this technique as a tool for aquatic biomonitoring. Hydrogen peroxide increased DNA single strand break with increasing concentration after 30 minutes exposure in vitro. Pyrene and benzo(a)pyrene caused DNA damage only at very high concentration (100 ㎍/L or 1000 ㎍/L) at two week exposure in vivo. DNA damage was relatively higher at hemocyte than at gill. It suggested that metabolized PAHs are transferred to hemolymph from digestive gland which have a relatively high enzyme activity, and attacked the DNA of hemocyre, while gill accumulated PAHs without degrading them to their metabolites due to low enzyme activity at gill. Both in vitro and in vivo exposure experiments showed that the comet assay is an effective tool on screening whether the organism are exposed to genotoxic contaminants. (rdf:langString) (en)
nlon:biographicalNote
  • 김기범, 경상대학교 해양환경공학과, 해양산업연구소, kgb (rdf:langString) (nongae.gsnu.ac.kr)
  • 배세진, 한국해양연구원 생태환경연구본부 (xsd:string)
nlon:classificationNumberOfNLK
  • 529.305 (xsd:string)
nlon:containedIn
dc:creator
  • 김기범 (xsd:string)
  • 배세진 (xsd:string)
dcterms:creator
nlon:datePublished
  • 2021-01-30T23:37:12 (xsd:dateTime)
dcterms:description
  • Use of the comet assay to assess DNA damage in hemocytes and gill of oyster(crassostrea gigas) exposed to pyrene and benzo(a)pyrene (xsd:string)
bibframe:extent
  • 30 cm (xsd:string)
  • p. 196-201 (xsd:string)
dcterms:isPartOf
nlon:issuedYear
  • 2003 (xsd:string)
nlon:itemNumberOfNLK
  • 한555ㅎ (xsd:string)
rdfs:label
  • Pyrene과 benzo(a)pyren에 노출된 굴의 혈구세포과 아가미 세포에서의 DNA 손상 측정을 위한 comet assay의 이용 / 김기범, 배세진 (xsd:string)
dcterms:language
dc:subject
  • Benzo(a)pyrene (xsd:string)
  • Comet assay (xsd:string)
  • Crassostrea gigas (xsd:string)
  • DNA damage (xsd:string)
  • Gill (xsd:string)
  • Hemocyte (xsd:string)
  • Oyster (xsd:string)
  • Pyrene (xsd:string)
  • (xsd:string)
  • 혈구세포 (xsd:string)
dcterms:title
  • Pyrene과 benzo(a)pyren에 노출된 굴의 혈구세포과 아가미 세포에서의 DNA 손상 측정을 위한 comet assay의 이용 (xsd:string)
rdf:type
nlon:volumeOfNLK
  • 16(1)-16(4) (xsd:string)