bibo:abstract
  • The nonstructural glycoprotein NSP4, encoded by the 10th gene of rotavirus, has been known to play important roles in viral assembly and pathogenesis. The NSP4 genes of human rotavirus Korean isolates, designated as CBNU/HR-1, CBNU/HR-2, CBNU/HR-3, and CBNU/HR-4, were cloned, sequenced and characterized. Also, the NSP4 gene of the CBNU/HR-1 was expressed in a baculovirus-insect cell system. The sequence data indicated that the NSP4 genes of human rotavirus Korean isolates were 750 or 751 bases in lenghth and encoded one open reading frame of 175 amino acids. Two glycosylation sites were recognized in the NSP4 gene of human rotavirus isolates tested. The NSP4 of CBNU/HR-1, CBNU/HR-3, and CBNU/HR-4 exhibited a high degree of amino acid sequence homology with that of NSP4 genotype B viruses, but a low degree of amino acid sequence homology with that of NSP4 genotype A viruses. However, the NSP4 of CBNU/HR-2 exhibited a high degree of amino acid sequence homology with that of NSP4 genotype A viruses, but a low degree of amino acid sequence homology with that of NSP4 genotype B viruses. The Sf9 cells infected with recombinant baculovirus, inserted with NSP4 gene of CBNU/HR-1, produced specific cytopathic effects and the expressed NSP4 was detected by immunofluorescence staining using NSP4-specific monoclonal antibody(MAb). The expressed NSP4 migrated at 16-26 kDa on SDS-PAGE and reacted with NSP4-specific MAb by Western blotting. (rdf:langString) (en)
nlon:biographicalNote
  • 강신영, 충북대학교 수의과대학/동물의학연구소, sykang (rdf:langString) (cbucc.chungbuk.ac.kr)
  • 김경미, 충북대학교 수의과대학/동물의학연구소 (xsd:string)
  • 박효선, 충북대학교 수의과대학/동물의학연구소 (xsd:string)
  • 백명순, 충북대학교 수의과대학/동물의학연구소 (xsd:string)
  • 송윤경, 충북대학교 수의과대학/동물의학연구소 (xsd:string)
  • 정동혁, (주)마이오인디스트 (xsd:string)
nlon:classificationNumberOfNLK
  • 528.05 (xsd:string)
nlon:containedIn
dc:creator
  • 강신영 (xsd:string)
  • 김경미 (xsd:string)
  • 박효선 (xsd:string)
  • 백명순 (xsd:string)
  • 송윤경 (xsd:string)
  • 정동혁 (xsd:string)
dcterms:creator
nlon:datePublished
  • 2021-01-30T23:37:12 (xsd:dateTime)
dcterms:description
  • Nucleotide sequence analysis and expression of NSP4 gene of human rotaviruses in isolated in Korea (xsd:string)
bibframe:extent
  • 26 cm (xsd:string)
  • p. 89-100 (xsd:string)
dcterms:isPartOf
nlon:issuedYear
  • 2002 (xsd:string)
nlon:itemNumberOfNLK
  • 대472 (xsd:string)
rdfs:label
  • 국내에서 분리된 사람 로타바이러스의 NSP4 유전자 염기서열 분석 및 발현 / 정동혁, 송윤경,김경미,박효선,백명순,강신영 (xsd:string)
dcterms:language
dc:subject
  • Baculovirus (xsd:string)
  • Expression (xsd:string)
  • Human rotavins (xsd:string)
  • NSP4 (xsd:string)
  • Nucleotide sequence (xsd:string)
  • 사람 로타바이러스 (xsd:string)
  • 유전자 염기서열 (xsd:string)
dcterms:title
  • 국내에서 분리된 사람 로타바이러스의 NSP4 유전자 염기서열 분석 및 발현 (xsd:string)
rdf:type
nlon:volumeOfNLK
  • 42(1) (xsd:string)