bibo:abstract
  • Eighteen embryonic stem(ES) cell lines (26%, 18/70) were established from 70 sheep whole blastocysts and isolated inner cell masses(ICM). The cells could be maintained undifferentiated on mouse fibroblast feed layer. The efficiency of isolation of ES-like colonies and established ES cell lines were higher (p〈0.05, by X² analysis) from isolated ICMs than from whole blastocysts. ES cell lines spontaneously differentiated into embryoid bodies in suspension culture, endoderm-like, muscular-like, neuron-like cells, lipid vesicle and stained positive for alkaline phosphatase activity. We are currently attempting to evaluate whether these cultures represent primary lineages which would be of particular relevance to developmental and transgenic studies. (rdf:langString) (en)
nlon:biographicalNote
  • Byoung-Gil Mheen, Genetic Resources Center, Korea Research Institute of Bioscience and Biotechnology (xsd:string)
  • Byung-Hwa Hyun, Genetic Resources Center, Korea Research Institute of Bioscience and Biotechnology (xsd:string)
  • Kyu-Shik Jeong, Genetic Resources Center, Korea Research Institute of Bioscience and Biotechnology (xsd:string)
  • Kyung-Kwang Lee, Genetic Resources Center, Korea Research Institute of Bioscience and Biotechnology (xsd:string)
  • Sun-Uk Kim, Genetic Resources Center, Korea Research Institute of Bioscience and Biotechnology (xsd:string)
  • Tae-Kwang Suh, Institute fo Human Infertility and Genetics, Kaya Mother and Child's Hospital (xsd:string)
  • Zae-Yoong Ryoo, Research Institute of Experimental Animals, Catholic Research Institutes of Medical Science, Catholic University Medical Collage (xsd:string)
nlon:classificationNumberOfNLK
  • 510.7205 (xsd:string)
nlon:containedIn
dc:creator
  • Hyun, Byung-Hwa (xsd:string)
  • Kim, Sun-Uk (xsd:string)
  • Lee, Kyung-Kwang (xsd:string)
  • Mheen, Byoung-Gil (xsd:string)
  • Ryoo, Zae-Yoong (xsd:string)
  • Suh, Tae-Kwang (xsd:string)
  • 정규식 (xsd:string)
dcterms:creator
nlon:datePublished
  • 2021-01-30T23:37:12 (xsd:dateTime)
dcterms:description
  • 면양 수정란을 이용한 배아주세포 확립 (xsd:string)
bibframe:extent
  • 28 cm (xsd:string)
  • p. 179-182 (xsd:string)
  • 삽도 (xsd:string)
dcterms:isPartOf
nlon:issuedYear
  • 1996 (xsd:string)
nlon:itemNumberOfNLK
  • 한547ㅎ (xsd:string)
rdfs:label
  • Efficiency of the establishment of sheep embryonic stem cell lines from whole blastocysts and isolated inner cell masses / Zae-Yoong Ryoo, Tae-Kwang Suh,Kyu-Shik Jeong,Sun-Uk Kim,Kyung-Kwang Lee,Byoung-Gil Mheen,Byung-Hwa Hyun (xsd:string)
dc:subject
  • Blastocysts (xsd:string)
  • Efficiency (xsd:string)
  • Embryoid body (xsd:string)
  • Embryonic stem cell line (xsd:string)
  • Establishment (xsd:string)
  • Inner (xsd:string)
  • Inner cell mass (xsd:string)
  • Isolated (xsd:string)
  • Masses (xsd:string)
  • Mouse fibroblast feed layer (xsd:string)
  • Sheep (xsd:string)
  • Whole (xsd:string)
  • 면양수정란 (xsd:string)
  • 배아주세포확립 (xsd:string)
dcterms:title
  • Efficiency of the establishment of sheep embryonic stem cell lines from whole blastocysts and isolated inner cell masses (xsd:string)
rdf:type
nlon:volumeOfNLK
  • 12(2) (xsd:string)