bibo:abstract
  • The expression levels and regional expressions of cytochrome P1501A1/2(CYP1A1/2) on F344 rat with treatments of diethylnitrosamine (DEN), partial hepatectomy (PH) and 2-acetylaminofluorene(2-AAF) was studied using immunohistochemical study. From 2 weeks until 8 weeks after intraperitoneal injection of DEN (200㎎/㎏), group Ⅰ (n=6) as a control was, given a basal diet after administration of saline instead of DEN and group Ⅱ (n=9) was given 0.004% in basal diet after administration of DEN as an experimental group. Both groups were subjected to two-thirds PH at 3 weeks after DEN or saline injection and then killed and fixed in 4% paraformal dehyde for paraffin embedded liver sections by studying of immunoreactivity of cytochrome P450 at week 8. AAF exposure for 8 weeks caused significant induction of the number (No./㎠) and area(mm/㎠) of GST p positive liver foci, a marker for single initiated cells and preneoplastic foci compared to untreated group. The average size of most types of foci was increased during continued AAF exposure of 8 weeks. Immunostaining of cytochrome P4501A1/2 from cosecutive thin sections from control and AAF treated liver revealed that hepatic CYP1A1/2 expression predominantly expressed in periportal hepatocytes. N-hydroxylation, and obligatory initial step in the activation of 2-AAF into electrophilic DNA binding metabolites is catalyzed predominantly be cytochrome P4501A1/2 enhanced protein aimed to protect the cell from further damage at the area of periportal hepatocytes. Differential localization of hepatocytes indicated that this regional selectivity is demonstrated heterogeneity of CYP1A1/2 expression and variation in expression may be of significance in assessing cell specific toxicities of various drugs and carcinogens. (rdf:langString) (en)
nlon:biographicalNote
  • Byoung-Gil Mheen, Genetic Resources Center, Korea Research Institute of Bioscience & Biotechnology, KIST, Taejon (xsd:string)
  • Byung-Hwa Hyun, Genetic Resources Center, Korea Research Institute of Bioscience & Biotechnology, KIST, Taejon (xsd:string)
  • Cha-Soo Lee, College of Veterinary Medicine, Kyoungpook National University, Taegu, Korea (xsd:string)
  • Chul-Ho Lee, Genetic Resources Center, Korea Research Institute of Bioscience & Biotechnology, KIST, Taejon (xsd:string)
  • Kyu-Shik Jeong, Genetic Resources Center, Korea Research Institute of Bioscience & Biotechnology, KIST, Taejon (xsd:string)
  • Ok-Seong Moon, Genetic Resources Center, Korea Research Institute of Bioscience & Biotechnology, KIST, Taejon (xsd:string)
  • Won-Kee Yoon, Genetic Resources Center, Korea Research Institute of Bioscience & Biotechnology, KIST, Taejon (xsd:string)
  • Yong-Kyu Choi, Genetic Resources Center, Korea Research Institute of Bioscience & Biotechnology, KIST, Taejon (xsd:string)
  • Zong-Zhu Yoon, Genetic Resources Center, Korea Research Institute of Bioscience & Biotechnology, KIST, Taejon (xsd:string)
nlon:classificationNumberOfNLK
  • 510.7205 (xsd:string)
nlon:containedIn
dc:creator
  • Choi, Yang-Kyu (xsd:string)
  • Hyun, Byung-Hwa (xsd:string)
  • Lee, Cha-Soo (xsd:string)
  • Lee, Chul-Ho (xsd:string)
  • Mheen, Byoung-Gil (xsd:string)
  • Yoon, Won-Kee (xsd:string)
  • Yoon, Zong-Zhu (xsd:string)
  • 문옥성 (xsd:string)
  • 정규식 (xsd:string)
dcterms:creator
nlon:datePublished
  • 2021-01-30T23:37:12 (xsd:dateTime)
dcterms:description
  • 랫드의 2-acettylaminofluorenen 에 의한 화학적 간장발암과정에서의 사이토크롬 P4501A1/2 에 대한 면역조직화학적 연구 (xsd:string)
bibframe:extent
  • 28 cm (xsd:string)
  • p. 61-67 (xsd:string)
  • 삽도 (xsd:string)
dcterms:isPartOf
nlon:issuedYear
  • 1996 (xsd:string)
nlon:itemNumberOfNLK
  • 한547ㅎ (xsd:string)
rdfs:label
  • Metabolic activation of 2-acetylaminofluorene is required for expression of dioxin receptor mediated cytochrome P4501A1/2 in hepatocarcinogenesis / Kyu-Shik Jeong, Chul-Ho Lee,Won-Kee Yoon,Zong-Zhu Yoon,Byoung-Gil MheenCha-Soo Lee,Yong-Kyu Choi,Ok-Seong Moon,Byung-Hwa Hyun (xsd:string)
dc:subject
  • 2-acetylaminofluorene (2-AAF) (xsd:string)
  • 2-acetylaminofluorenen (xsd:string)
  • Activation (xsd:string)
  • Cytochrome (xsd:string)
  • Cytochrome P4501A1/2 (CYP1A/2) (xsd:string)
  • Diethylnitrosamine (DEN) (xsd:string)
  • Dioxin (xsd:string)
  • Expression (xsd:string)
  • Hepatocarcinogenesis (xsd:string)
  • Mediated (xsd:string)
  • Metabolic (xsd:string)
  • P4501A1/2 (xsd:string)
  • Partial hepatectomy (PH) (xsd:string)
  • Receptor (xsd:string)
  • Required (xsd:string)
  • 간장발암과정 (xsd:string)
  • 랫드 (xsd:string)
  • 면역조직화학 (xsd:string)
  • 사이토크롬 (xsd:string)
  • 화학적 (xsd:string)
dcterms:title
  • Metabolic activation of 2-acetylaminofluorene is required for expression of dioxin receptor mediated cytochrome P4501A1/2 in hepatocarcinogenesis (xsd:string)
rdf:type
nlon:volumeOfNLK
  • 12(1) (xsd:string)